A SPERM SELECTION TECHNIQUE TO IMPROVE EMBRYO PLOIDY

To assess the role of an enhanced sperm selection method in mitigating paternal contributions to embryo aneuploidy. Over last 4 years, 57 couples underwent ICSI with selected by density gradient centrifugation (DGC), resulting few frozen transfers (FETs) due consistent aneuploidy following preimplantation genetic testing for (PGT-A). These men consented chromatin fragmentation (SCF) assessment, inclusive double-stranded DNA breaks (dsDNA) their raw semen, as well post-DGC and post-microfluidic (MFSS). subsequent cycles MFSS. Outcomes processed DGC MFSS were analyzed compared. SCF was assessed terminal deoxynucleotidyl dUTP transferase nick-end labeling (TUNEL) on ≥500 spermatozoa per patient, a normal threshold ≤15%. A neutral Comet assay used dsDNA ≥200 spermatozoa, utilizing modified in-house protocol ≤3%. total had semen parameters: concentration 40.0±32x106/mL, 37.1±11% motility, 2.2±1% morphology. After or MFSS, concentrations 3.3±3.4 8.0±13x106/mL, 58.0±29% 96.9±9% respectively (P<0.0001). The decreased from 21±14% specimens 18±6% 1.9±1% (P<0.001). fell 3.6±2% 3.1±1% after 0.3±0.2% (40.9±6 years) 71 female partners (36.5±5 years), achieving fertilization rate 58.4% (403/690) blastocyst euploidy 28.5% (47/165). Only 19 FET performed, 6.7% implantation (2/30) two clinical pregnancies miscarriage. Subsequently, these cycles, higher 75.9% (647/852; P<0.0001) much improved (P<0.0001) 48.9% PGT-A (192/389). In 48 51 embryos replaced increased 60.8% (31/51; P<0.0001), CPR 64.6% (31/48; P<0.001), ongoing/delivery 62.5% (30/48; P<0.0001). With its component, tangibly contributes structural chromosomal abnormalities. An appears remarkably increase proportion euploid blastocysts consequent successful outcomes.